Table 4.
PCR analysis of total RNA from liver, brain, and injured muscle of TO-treated animals. Comparison of acute-phase cytokines (IL1β, IL6 and, TNF-α) in the rat liver, brain, and injured muscle at various time points after intramuscular TO injection in comparison with control animals. Data show the results of three animals (mean values±SEM)
| Time (h) | IL-1β mRNA expression | IL-6 mRNA expression | TNF-α mRNA expression | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Injured muscle | Liver | Brain | Injured muscle | Liver | Brain | Injured muscle | Liver | Brain | |
| Control | 1.0±0.00 | 1.0±0.00 | 1.0±0.00 | 1.0±0.1 | 1.0±0.3 | 1.0±0.3 | 1.0±0 | 1.0±0.3 | 1.0±0.3 |
| 0.5 | 1.4±0.3 | 1.6±0.01 | 0.5±0.01 | 0.8±0.3 | 1.2±0.5 | 0.5±0.1 | 0.7±0.3 | 0.8±0.1 | 0.3±0.01 |
| 1 | 1.1±0.2 | 1.8±0.1 | 0.4±0.1 | 0.2±0.1 | 0.5±0.1 | 0.8±0.1 | 0.3±0.1 | 1.0±0.2 | 0.9±0.2 |
| 2 | 28±6 | 1.1±0.01 | 0.3±0.01 | 147±582 | 0.3±0.1 | 1.2±0.2 | 1.1±0.4 | 1.2±0.1 | 0.4±0.1 |
| 4 | 355±80 | 3±0.4 | 0.5±0.2 | 1055±414 | 0.5±0.1 | 1.9±0.4 | 3.6±1.5 | 2.4±0.2 | 0.2±0.01 |
| 6 | 425±95 | 4.5±0.6 | 0.5±0.1 | 1982±777 | 0.4±0.1 | 2.3±0.4 | 2.2±0.9 | 4.0±1.0 | 1.3±0.3 |
| 12 | 299±67 | 3.1±0.4 | 0.8±0.1 | 371±61 | 1±0.2 | 0.4±0.01 | 4.0±1.1 | 2.1±0.6 | 2.3±0.1 |
| 24 | 53±12 | 0.8±0.1 | 0.6±0.4 | 27±19 | 1.7±0.4 | 0.7±0.04 | 3.2±2.6 | 1.2±0.04 | 0.8±0.1 |
| 36 | 23±5 | 1.8±0.3 | 0.4±0.2 | 109±43 | 0.4±0.1 | 0.4±0.01 | 1.4±0.6 | 1.4±0.01 | 0.8±0.01 |
| 48 | 80±18 | 1.4±0.4 | 0.9±0.2 | 75±30 | 1.2±0.3 | 0.6±0.02 | 3.6±3.0 | 1.4±0.02 | 0.9±0.2 |