Fig. 4.

VAMP8 deficiency leads to altered distribution of cortical thymic epithelial cells (cTEC) and medullary thymic epithelial cells (mTEC). mTEC were identified by keratin 5 (red) and cTEC by keratin 8 (green) immunostaining. Both cell populations were present in a small and healthy Vamp8^−/− mouse (8 days old, a–b’’). However, their distribution was scattered and disorganized in comparison with a Vamp8^+/− control littermate (c–d’’) and a wild-type mouse (8 days old, e–f’’). Fluorescence micrographs taken at a magnification of ×100, bars 50 µm (a, c, e); enlargements of boxed areas taken at ×400, bars 10 µm (b, d, f)