Fig. 6.

VAMP8 deficiency led to a disturbance of DN thymocytes. A flow cytometric analysis of the DN precursor subsets in thymus of small and sick Vamp8^−/− mice was performed. Lymphocytes were gated according to their light scattering characteristics, and the lineage marker negative cells were gated in a second step. The DN cells were further separated into DN1 to DN4 subsets depending upon the intensity of staining for CD44 and CD25 markers. A distribution plot shows a summary of six independent experiments (age: 10–12 days) comparing the DN1 (a), DN2, (b), DN3 (c), and DN4 (d) subsets of small and sick Vamp8^−/− mice and Vamp8^+/− littermates