Table 2.
Effects of testosterone propionate (TP) and endurance training (Tr) on activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR) in the left ventricle of young male Wistar rats
| Enzyme | UTr | UTr + TP8 | UTr + TP80 | Tr | Tr + TP8 | Tr + TP80 | Two-way ANOVA results | Spearman rank correlation with serum total testosterone level | |
|---|---|---|---|---|---|---|---|---|---|
| R | P | ||||||||
| SOD [U/mg protein] | 3.93 (0.63) | 3.30 (0.39) | 1.57***††† (0.54) | 5.10### (0.59) | 2.79*** (0.22) | 0.68***##††† (0.23) |
Training: F 1,42 = 0.33, P = 0.57 TP treatment: F 2,42 = 215.4, P < 10−6 Interaction: F 2,42 = 22.1, P < 10−6 |
−0.82 | <10−6 |
| CAT [1/(s × g protein)] | 11.38 (1.48) | 13.75 (2.70) | 7.40†† (1.35) | 20.92### (5.93) | 11.73*** (3.19) | 6.70***† (1.07) |
Training: F 1,42 = 6.43, P = 0.015 TP treatment: F 2,42 = 35.1, P < 10−6 Interaction: F 2,42 = 16.6, P = 5×10−6 |
−0.71 | <10−6 |
| GPX [mU/mg protein] | 263.3 (60.3) | 260.2 (24.1) | 213.7* (14.1) | 279.1 (22.5) | 256.9 (27.2) | 181.1***###††† (14.6) |
Training: F 1,42 = 0.54, P = 0.46 TP treatment: F 2,42 = 25.4, P < 10−6 Interaction: F 2,42 = 2.4, P = 0.10 |
−0.57 | 0.0003 |
| GR [mU/mg protein] | 5.09 (0.78) | 4.39 (0.32) | 3.84*** (0.85) | 4.48 (0.30) | 3.56# (0.21) | 3.14*** (0.33) |
Training: F 1,42 = 21.5, P = 3×10−5 TP treatment: F 2,42 = 24.7, P < 10−6 Interaction: F 2,42 = 0.19, P = 0.83 |
−0.62 | <10−4 |
For group labels, see Table 1. Data are means (SD). * P < 0.05, *** P < 0.001 vs. the respective TP-untreated group value; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the respective untrained group value; † P < 0.05, †† P < 0.01, ††† P < 0.001 vs. the respective low TP dose-treated group value