Table 3.
Effects of testosterone propionate (TP) and endurance training (Tr) on nonenzymatic antioxidants’ levels in the left ventricle of young male Wistar rats
| Antioxidant | UTr | UTr + TP8 | UTr + TP80 | Tr | Tr + TP8 | Tr + TP80 | Two-way ANOVA results | Spearman rank correlation with serum total testosterone level | |
|---|---|---|---|---|---|---|---|---|---|
| R | P | ||||||||
| GSH [μmol/g tissue] | 1.90 (0.21) | 1.76 (0.16) | 1.68 (0.22) | 1.54## (0.19) | 1.74 (0.76) | 1.49 (0.28) |
Training: F 1,42 = 11.1, P = 0.002 TP treatment: F 2,42 = 3.06, P = 0.058 Interaction: F 2,42 = 2.90, P = 0.066 |
−0.005 | 0.76 |
| Protein thiols [μmol -SH/g protein] | 218.1 (10.5) | 256.3** (22.6) | 233.1 (9.0) | 200.7 (22.4) | 247.2** (25.2) | 205.6†† (28.7) |
Training: F 1,42 = 8.75, P = 0.005 TP treatment: F 2,42 = 17.7, P = 3×10−6 Interaction: F 2,42 = 0.77, P = 0.47 |
0.23 | 0.17 |
| α-tocopherol [μmol/g tissue] | 0.0541 (0.0043) | 0.0358*** (0.0040) | 0.0438***† (0.0043) | 0.0480 (0.0056) | 0.0339*** (0.0051) | 0.0379*** (0.0030) |
Training: F 1,42 = 12.7, P = 0.0009 TP treatment: F 2,42 = 53.9, P < 10−6 Interaction: F 2,42 = 1.10, P = 0.34 |
−0.49 | 0.0025 |
| γ-tocopherol [μmol/g tissue] | 0.00120 (0.00014) | 0.00149 (0.00033) | 0.00161 (0.00035) | 0.00088 (0.00019) | 0.00144** (0.00046) | 0.00129 (0.00068) |
Training: F 1,42 = 6.97, P = 0.012 TP treatment: F 2,42 = 10.1, P = 0.0003 Interaction: F 2,42 = 1.07, P = 0.35 |
0.35 | 0.038 |
For group labels, see Table 1. Data are means (SD). ** P < 0.01, *** P < 0.001 vs. the respective TP-untreated group value; ## P < 0.01 vs. the respective untrained group value; † P < 0.05, †† P < 0.01 vs. the respective low TP dose-treated group value