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. 2008 Nov 7;18(6):857–866. doi: 10.1089/scd.2008.0291

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 5. — Detection of embryonic markers Oct-3/4 and Nanog. Mesodermal progenitors cells (MPCs) appear rounded and with not organized globular actin (red in A and C). Furthermore, cells show a bright positive stain for Oct-3/4 (A) and Nanog (C) into the nucleus. The nuclear localization of green fluorescence is confirmed by confocal microscopy in spectral mode (B and D) where nuclei of MPCs are identified by negative CD90 stain (arrows). In contrast, co-cultured MSCs show typical spindle-shaped morphology and well-organized F-actin cytoskeleton (A and C). MSCs' nuclei are negative for Oct-3/4 and Nanog. DAPI: 4′,6-diamidino-2-phenylindole. RT-PCR analysis (E) reveals, in all three assays performed, high expression of OCT3/4 on MPCs and low level on trypsin-detached cells (MSCs), while NANOG is exclusively expressed by MPCs.