Figure 4.

Thiamine acts on the BRCS and CCE in the presence or absence of oxidants.

a. Thiamine diminished BRCS in the presence or absence of t-BHP, but did not affect CCE in the presence or absence of t-BHP. Fibroblasts were loaded with Fura 2 (2 µM) for 60 min. After loading, the media were changed to calcium free BSS with or without 1 mM-thiamine, and 3 min later the calcium measurements were initiated in the presence or absence of thiamine. t-BHP (100 µM) was added after 1 min basal [Ca²⁺]_i measurement, and bombesin (1 µM) was added 3 min after t-BHP treatment. After an additional 3 min, CPA (2 µM) was added, and 3 min later CaCl₂ (2.5 mM) was added. The top panel shows the tracings taken from 94–113 cells. The bottom panel shows the integration of the [Ca²⁺]_i peak over the 1 min interval after calcium addition. Data are means ± SEM (n = 94–113 cells). Values for BRCS or CCE with different letters vary significantly (p<0.05) from each other by ANOVA followed by Student Newman Keul’s test.

b. Thiamine diminished the exaggeration of CCE by SIN-1. Fibroblasts were loaded with Fura 2 (2 µM) for 60 min. After loading, the media were changed to calcium free BSS with or without thiamine, and 3 min later the calcium measurements were initiated in the presence or absence of thiamine. SIN-1 (500 µM) was added after 1 min basal [Ca²⁺]_i measurement, and bombesin (1 µM) was added 3 min after SIN-1 treatment. After an additional 3 min, CPA was added, and 3 min later CaCl₂ (2.5 mM) was added. The top panel shows the tracings taken from 79–128 cells. The bottom panel shows the integration of the [Ca²⁺]_i peak over the 1 min interval after bombesin or calcium addition. Data are means ± SEM (n= 79–128 cells). Values for BRCS or CCE with different letters vary significantly (p<0.05) from each other by ANOVA followed by Student Newman Keul’s test.