Figure 4. FAK transduces signals in migrating cells.
A) Immunoblot blot analysis of IOMM-Lee and CH-157-MN cell lysates treated with radiation (7 Gy). We checked for FAK and pFAK (Tyr 397), and GAPDH served as a loading control. B) The relative phosphorylation was quantified. C) IOMM-Lee and CH-157-MN cells (1×105) cells were seeded in 8-µm pore size transwell inserts, irradiated, treated with FAK inhibitor as indicated, and allowed to migrate for 24 hrs. Cells that remained in the upper chamber and those that attached to the other side were fixed, stained and counted under a light microscope. The percent of migrated cells was calculated. Western blotting analysis of IOMM-Lee and CH-157-MN cell lysates treated with 10 µM FAK phosphorylation inhibitor followed by irradiation (7 Gy). GAPDH served as a loading control. C) Western blotting analysis of the IOMM-Lee and CH-157-MN cell lysates transfected with FAK shRNA for 24 hrs and treated with 7 Gy. GAPDH served as a loading control. All blots are representatives of three independent experiments. Values are mean ± S.D. from three independent experiments. * Statistically different (P<0.01)