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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: J Immunol. 2010 Mar 15;184(8):4185–4195. doi: 10.4049/jimmunol.0903420

FIGURE 4.

FIGURE 4

Human B lymphocytes express lower levels of Siglec-5, which correlate with increased calcium flux and higher proliferative responses. A, Siglec-5 expression on CD19+ lymphocytes was analyzed in freshly isolated PBMCs by flow cytometry. Data shown are the average of the mean fluorescence intensity from cells from four individuals from each species. Error bars represent SD. B, PBMCs were labeled with calcium sensitive dyes (Fluo-4/FuraRed) and stimulated with ionomycin (1 mM), anti-IgD (5 μg/ml), and anti-IgM (5 μg/ml) Abs. The relative change in calcium levels was recorded in CD19+ cells using a flow cytometer. One experiment representative of three comparisons is shown. C, PBMCs labeled with CFSE were stimulated with anti-IgD, anti-IgM, and anti-CD3/CD28 Abs for 5 d. The amount of proliferation in CD19+ cells was measured by flow cytometry. One experiment representative of three comparisons is shown.