Figure 8.

TLR4 triggers inflammatory cytokine release and promotes IRAK modulated p47^phox phosphorylation in response to oxidized phospholipid treatment in BMDM derived from TLR4^wt and TLR4^d mice. (A) BMDM were treated with PAPC and oxPAPC and inflammatory cytokine levels in the supernatant were determined. (n=3/group; *p<0.05) (B) These blots show p47^phox expression and phosphorylation in response to PAPC and oxidized PAPC treatment. Lysates from bone marrow derived monocytes isolated from TLR4^wt and TLR4^d mice were immunoblotted for p47^phox and phospho-p47^phox (left). A subset of experiments was performed in presence of an IRAK inhibitor. The figure on the right represents the photodensitometric quantification of the blots. (n=3) Data are mean ± SD. (*p<0.05).