TABLE 4.

Identified HLA-A0201 and HLA-A0101 Binding Mesothelin Epitopes

Peptide Number	Amino Acid Position	Peptide Sequence	HLA-restriction
31D	153–161	RGAPERQRL	HLA-A0101
32D	158–166	RQRLLPAAL	HLA-A0101
36A	175–183	LLSEADVRA	HLA-A0101
37C	182–190	RALGGLACD	HLA-A0101
44C	217–225	DQQEAARAA	HLA-A0101
45E	224–232	AALQGGGPP	HLA-A0101
16F	80–88	RVRELAVAL	HLA-A0201
32E	159–167	QRLLPAALA	HLA-A0201
44F	220–228	EAARAALQG	HLA-A0201
45D	223–231	RAALQGGGP	HLA-A0201

Peripheral blood leukocytes from 2 vaccinated participants demonstrating prolonged disease-free survival were used to screen an overlapping mesothelin peptide library. Shown are the amino acid positions and sequences of the 6 newly identified HLA-A0101 binding (top) and 4 newly identified HLA-A0201 binding (bottom) mesothelin-derived 9mer epitopes.