Figure 4.

Bone marrow reconstitution shows a strong effect of cardiac CyPA for recruitment of bone marrow-derived cells in response to AngII, and development of cardiac hypertrophy. GFP⁺ bone marrow cells (Ppia^+/+) were transplanted into irradiated Apoe^−/− or Apoe^−/− Ppia^−/− mice. After 6 weeks, these chimeric mice with GFP⁺ bone marrow were infused with saline (A–B) or AngII for 4 weeks (C–D). (A–D) Representative PECAM-1 staining (red) of hearts from Apoe^−/− and Apoe^−/− Ppia^−/− recipient mice with GFP⁺ bone marrow (green). Statistical analysis of the number of migrating GFP⁺ bone marrow in the cardiac tissue (E) or perivascular area (F) in the hearts of Apoe^−/− (n = 9) and Apoe^−/− Ppia^−/− (n = 8) mice. (G) AngII-induced cardiac hypertrophy (heart weight per body weight ratio) was significantly less in Apoe^−/− Ppia^−/− recipient mice (n = 9) compared with Apoe^−/− recipient mice (n = 8) with Ppia^+/+ bone marrow. (H) Heart weight per body weight ratio after AngII infusion for 4 weeks, was significantly less in ApoE^−/− Ppia^−/− (n = 6) compared to ApoE^−/− (n = 6) chimeric mice with Ppia^−/− bone marrow. Results are mean ± SD. # equals P< 0.05 in Saline versus AngII; *equals P< 0.05 in Apoe^−/− versus Apoe^−/− Ppia^−/− mice.