Figure 2. Comparison of EGF- and scFv-adapter-mediated EGFR-dependent HSV infection of HSV-resistant cells.
EGFR-positive or -negative CHO-K1 (A, C) and J1.1-2 cells (B, D) were incubated for 2 h at 37°C with scFv adapter, EGF adapter, or no adapter and a replication-defective HSV-1 recombinant virus expressing LacZ from the immediate-early ICP0 promoter (QOZHG; MOI=3). Cells were washed after 2 h, incubated with fresh media for 16 h, and examined for LacZ activity by X-gal staining (A, B) or quantitative ONPG assay (average of triplicate samples) (C, D).