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Enhancement of leishmanicidal activity of ASM by PLL in axenic amastigotes. Growing cultures of the axenic amastigotes (~5 × 106 cells/mL) were incubated with 10 µM SSM or ASM for 20 hr with or without PLL (10 µg/mL). Results are means ± SEM (N = 6). In the presence of PLL (10 µg/mL), 20 µM ASM completely lysed the amastigotes of all three species. The differences between SSM and ASM, between SSM and ASM + PLL, and between ASM and ASM + PLL were statistically significant (P < 0.01).
