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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Biochem J. 2009 Jun 26;421(2):311–322. doi: 10.1042/BJ20090039

Figure 2. Acyl-CoA thioesterase activity of Them2.

Figure 2

(A) The time-dependent Them2-mediated hydrolysis of 100 µM myristoyl-CoA was monitored at A412 for various Them2 concentrations: 0 µM (●), 1.7 µM (○), 3.3 µM (■), 6.6 µM (□), 13.2 µM (▲), 19.8 µM (△), 26.4 µM (◆) and 33.0 µM (◇). (B) The time-dependent hydrolysis by 5.0 µM Them2 of varied myristoyl-CoA concentrations: 0 µM (●), 10 µM (○), 20 µM (■), 40 µM (□), 60 µM (▲), 80 µM (△), 100 µM (◆) and 120 µM (◇). Reactions were carried out at 37°C in mixtures that included 10 mM Hepes (pH 7.5), 50 mM KCl and 0.3 mM DTNB. Each data point represents the average of two A412 determinations.