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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Biochem J. 2009 Jun 26;421(2):311–322. doi: 10.1042/BJ20090039

Figure 7. Tissue distribution, subcellular localization and PPARα-mediated regulation of Them2.

Figure 7

(A) Them2 tissue distribution was ascertained by Western blot analysis of tissue homogenates harvested from two FVB/NJ mice (40 µg of protein/lane). The blot was also probed with an anti-β-actin antibody. (B) Subcellular localization of Them2 and StarD2 was determined by Western blot analysis of proteins (40 µg/lane) from liver homogenate (L), nuclei (N), cytosol (C), mitochondria (M) and endoplasmic reticulum (E) following purification from the liver of an FVB/NJ mouse. (C) Regulation of Them2 mRNA by PPARα was ascertained using FVB/NJ mice (n = 8/group), as well as wild-type (n = 4/group) and Ppara−/− 129S3/ SvImJ (n = 6/group). Mice were fed on chow (closed bars) or chow supplemented with 0.2% fenofibrate (open bars) for 7 days. Them2 mRNA was quantified by real-time PCR using cyclophilin as an invariant reference gene. The expression in chow-fed wild-type mice was set at 100%. Results are means +S.E.M. *P < 0.05, using a two-tailed Student’s t test.