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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Dev Cell. 2011 Apr 19;20(4):419–429. doi: 10.1016/j.devcel.2011.03.012

Figure 2. DNA methylation is required for maintenance of beta cell identity.

Figure 2

(A) Glucose tolerance test (GTT) for RC: Dnmt1fl/+ (control) and RC:Dnmt1fl/fl animals at ages 3 months (left panel) and 6 months (right panel) (n=5 for each genotype). (B) Representative pancreatic section from an 8 months old RC:Dnmt1fl/fl:R26RLacZ animal showing immunostaining for glucagon (green) in the left panel and an overlay of glucagon with beta galactosidase (b-gal; red), in the middle panel. The inset marks a representative area, magnified and shown in right panel. A number of beta-galactosidase and glucagon double labeled cells (white arrows) are evident in these RC:Dnmt1fl/fl:R26RLacZ animals. Green arrows indicate normal alpha cells, which express glucagon and do not stain for beta galactosidase. (C) Quantification of endocrine cells co-staining for glucagon and RIP-Cre driven beta-galactosidase in RC: Dnmt1fl/+:R26RLacZ (control) and RC:Dnmt1fl/fl:R26RLacZ animals at 4 days and 8 months of age. N=3 animals per group. The error bars represent standard error (SEM) of the mean. See also Figure S2.