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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Virology. 2011 Jan 14;411(1):65–77. doi: 10.1016/j.virol.2010.12.038

Figure 2. Vpu-A18H inefficiently enhances virion release.

Figure 2

(A) Cells (HeLa) were transfected with either a proviral plasmid expressing wild type HIV-1NL4-3 or a vpu-negative proviral plasmid vpuDEL-1 (ΔVpu) (1.6μg). Each of the Vpu mutant-proteins was expressed in trans (160ng) with ΔVpu to assess their activity in virion release. The next day, the fraction of the total p24 capsid antigen produced by the cells that was secreted into the media was measured by ELISA. The average values from two independent experiments are graphed; the error bars indicate the actual values obtained from each experiment. (B) Cells were transfected with plasmid expressing the indicated codon-optimized Vpu (160ng). The next day cells were harvested and the cell lysates were analyzed by immunoblot for Vpu, BST-2, and β-actin.