Table 1. Sequences of primers used in this study.
Primers for preparation of standards begin with a GCG clamp, followed by either a Hind III site (for forward primers) or an Xba I site (for reverse primers). Sizes of amplicons are indicated; for UTR primers, predicted and (actual) sizes are presented. F, forward primer; R, reverse primer.
| RefSeq | Purpose | Primers | Size (nt) |
|---|---|---|---|
| XM_001471624 | qRT-PCR | F:AGACCAAGGGAGATCCG | 117 |
| R:ATATGAGTCGTGGTCCCTCT | |||
| Exon 2 standard | F:GCGAAGCTTGAACAAACTGGTTGGTTGAGATTGTCTGCTTGATTCAGACCAAGGGAGATCCG | 209 | |
| R:GCGTCTAGACTTGGCCTTGGAACTGAAGAAAGACTTGGAGAAGAGATGAATGGGAAGATGGGAGGATATGAGTCGTGGTCCCTCT | |||
| UTR | F: CCTGGACTGGAATC | 982 (982) | |
| R: TATGACCATATTCAGGTA | |||
| NM_009286 | qRT-PCR | F:AACTGGCTGAATGAGATTGTA | 138 |
| R:GTGGTCCTTCCTTATTGATTAT | |||
| Exon 2 standard | F:GCGAAGCTTGAACGAACTGGCTGAATGAGATTGTA | 209 | |
| R:GCGTCTAGACTTGGCCTTGGAACTGAAGAAAGACTTGGAGAAGAGATGGATGGGAAGATGGGAGGTTATGAGTCGTGGTCCTTCCTTATTGATTAT | |||
| UTR | F: GGCTGGAATCCTAAG | 978 (705) | |
| R: TATGACCATATTCAGGTA | |||
| NM_001111296 | qRT-PCR | F:AACTGTGCCCATTTGGA | 80 |
| R:GTGGTCCTTCCTTATTGATTAA | |||
| Exon 2 standard | F:GCGAAGCTTGAACGAACTGGCTGATTGAGATTGTATGCTTGATTCAGACCAAGGGAGATCCGAAGTGGATCCAAACTGTGCCCATTTGGA | 209 | |
| R:GCGTCTAGACTTGGCCTTGGAACTGAAGAAAGACTTGGAGAAGAGATGGATGGGAAGATGGGAGGTTATGAGTCGTGGTCCTTCCTTATTGATTAA | |||
| UTR | F: GGCTGGAATCCTAAGA | 972 (972) | |
| R: CCACATCTAGGTATTCAAGA | |||
| NM_001101534 | qRT-PCR | F:ATCCAAACTGTGTCCATTCA | 109 |
| R:TGGGAAGATGGGAGGTTA | |||
| Exon 2 standard | F:GCGAAGCTTGAACAAACTGGTTGATTGAGATTGTCTGCTTGATTCAGACCAAGGGAGATTCAAAGTGGATCCAAACTGTGTCCATTCA | 209 | |
| R:GCGTCTAGACTTGGCCTTAGAACTGAAGAAAGACTTGGAGAAGAGATGGATGGGAAGATGGGAGGTTA | |||
| UTR | F: CTGGGCTGGAATTCT | 988 (988) | |
| R: GGATTTATTCTATGACCACA | |||
| XM_894052 | qRT-PCR | F:CTTGATTCAGACCAGG | 155 |
| R:TGAAGAAGAGATGGACA | |||
| Exon 2 standard | F:GCGAAGCTTGAACACACTGGCTGATTGAGATTGTCTGCTTGATTCAGACCAGG | 209 | |
| R:GCGTCTAGACTTGGCCTTGGAACTGAAAAAAGACTTGAAGAAGAGATGGACA | |||
| UTR | F: GTCTTCGGCTGGAATT | 971 (971) | |
| R: CAGGTGTTCAAGATACAGAA | |||
| NM_001081325 | qRT-PCR | F: AGATTGTCTGCTTGATTCAG | 125 |
| R: GTGGTCCTTCCTTACTGG | |||
| Exon 2 standard | F:GCGAAGCTTGAACAAACTGGCTGATTGAGATTGTCTGCTTGATTCAG | 209 | |
| R:GCGTCTAGACTTAGCTTTGGAACTGAAGAAAGACTTGGAGAAAAGATGGATGGGAAGATGGGAGGTCATGAGTCGTGGTCCTTCCTTACTGG | |||
| UTR | F: GGTCAGGGCTGGAAC | 981 (981) | |
| R: TATTCTATGACCGCATTCAA | |||
| XM_983034 | qRT-PCR | F:TGATTCAGAAGAAGGGAGATAC | 170 |
| R:TGGAACTAAAGAGAGACTTGTG | |||
| Exon 2 standard | F:GCGAAGCTTGAATGACCTGGCTTGTTGAGATTGTCTGCTTGATTCAGAAGAAGGGAGATAC | 209 | |
| R:GCGTCTAGACTTGGTCTTGGAACTAAAGAGAGACTTGTG | |||
| UTR | F: CATCAGACTACGTTTGGT | 1003 (738) | |
| R: TTGTACAAGAACTGGAAAAT | |||