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Nickel ion binding by Mua protein based on equilibrium dialysis. Purified Mua protein (3 to 4 µM dimer concentration) in 50 mM NaCl (pH 8.25) was equilibrated with the indicated concentrations of NiCl2, and the number of nickel ions bound per dimer was determined by graphite furnace atomic absorption spectrophotometry. Standard deviations were ≤10% for each set of measurements. SigmaPlot, version 11, was used to generate the graph, as well as to calculate the KD for Ni (50% saturation).
