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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Eur J Immunol. 2010 Sep;40(9):2580–2589. doi: 10.1002/eji.201040540

Figure 1.

Figure 1

Alpha7nAChR inhibits STAT3 tyrosine phosphorylation. WT or alpha7nAChR-knockout (α7KO) mice were treated with (A) nicotine (0.5 mg/Kg) or (B) choline (10 mg/kg; i.p.) 30 min prior to LPS (6 mg/kg; i.p.). TNF levels in serum were quantified by ELISA at 90 min after LPS. *p<0.01 versus LPS(n = 4/group; One-way ANOVA with Bonferroni's corrections). (C) Splenocytes from WT and alpha7nAChR-knockout mice were treated with choline 30 min before treatment with LPS (100 ng/mL). (D) RAW264.7 cells were treated with choline alone or with AG490 for half an hour before LPS, and TNF levels were quantified 3 h later. Upper panel represents cell survival determined by MTT assay. *p<0.01 versus LPS (n = 3/group; One-way ANOVA with Bonferroni's corrections).(E) Human THP1 cells were pretreated with choline (3h) and STAT3 phosphorylation in tyrosine (705) and serine (727) was determined by Western blot. The left panel shows a representative Western blot of three different experiments. Total STAT3 was used as a loading control. The films were scanned and the densitometric data were represented as mean±SD (n = 3) in the right panel.