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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Eur J Immunol. 2010 Sep;40(9):2580–2589. doi: 10.1002/eji.201040540

Figure 2.

Figure 2

JAK2 inhibition halts STAT3 phosphorylation and cytokine production. (A) RAW264.7 cells were treated with LPS for 3 hs, and STAT3 phosphorylation was analyzed by Western blot using total STAT3 protein as a loading control. The upper panel shows a representative, whereas the lower panel shows the densitometric data of three different experiments (mean±SD, normalized as percent of the control treatment). (B) AG490 inhibited LPS-induced STAT3 phosphorylation in tyrosine (705). RAW264.7 cells were pretreated with JAK2 inhibitor (AG490) 30 min prior to LPS stimulation. The upper panel shows a representative Western blot, whereas the lower panel represents the densitometric data of three different experiments (mean±SD, normalized as percent of the LPS treatment). Cells were treated with AG490 half an hour before LPS on (C) RAW264.7 or (D) THP1 cells. TNF levels were determined by ELISA at 3 h posttreatment. Upper panel represents cell survival as determined by MTT assay. *p<0.01 versus LPS (n = 3; One-way ANOVA with Bonferroni's corrections).