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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Eur J Immunol. 2010 Sep;40(9):2580–2589. doi: 10.1002/eji.201040540

Figure 5.

Figure 5

STAT3 inhibition controls inflammation and rescues animals in sepsis. (A) Endotoxemic animals were treated with vehicle or the STAT3 inhibitor stattic (25 mg/kg), 30 min before LPS challenge. TNF levels in the samples were normalized to total protein (*p<0.01 versus LPS; n = 6/group). (B) BALB/c mice underwent sham surgery or splenectomy 5 days before LPS challenge. Animals were treated with stattic, and serum TNF levels were analyzed by ELISA. (C) Animals were treated with vehicle, 10 or 25 mg/kg stattic, 30 min prior to endotoxin. Animals were followed for 2wk (*p<0.01 versus control, n = 10/group Logrank Test). (D) RAW264.7 macrophages were treated with stattic and HMGB1 levels were analyzed by Western blot in the extracellular media (*p<0.01 versus LPS; n = 3 experiments; ANOVA with Bonferroni's modifications). (E) Animals were treated with vehicle or stattic and systemic HMGB1 levels were determined at 24 h. (*p<0.01 versus LPS; n = 6 mice/group; One-way ANOVA with Bonferroni's corrections). (F) BALB/c mice underwent CLP and the stattic treatment (25 mg/kg; i.p.) was started 24 h later. Animals were observed for 2 wk. (*p<0.01 versus control, n = 10 mice/group; Logrank Test).