Fig. 6.
Radio TLC of supernatant resulting from centrifugation filtration of SLNs incubated with saline (pH 2) and saline (pH 4) at 3 and 20 hr. Samples were developed on a silica plate was developed with methanol/ammonium acetate (10%) (50:50, v/v) and recorded by a radio-TLC Imaging Scanner. The TLC plots confirm that the loss of radiolabel observed (Figure 4) for 64Cu-SLNs incubated with saline (pH 2) and saline (pH 4) were a result of 64Cu-EDTA dissociating from the particle in these extreme conditions.