Fig. 7.

Levels of proapoptotic gene expression are increased throughout development in dmt mutants and fail to decrease in irradiated late stage dmt embryos. A–X: Transcript levels of the proapoptotic genes hid and rpr are significantly higher in dmt mutants (C,G,K,O,S,W) than in wild-type (A,E,I,M,Q,U) at all stages of embryonic development. At early stages, hid and rpr levels are higher in irradiated wild-type and dmt embryos (B,D,N,P) than in nonirradiated embryos (A,C,M,O). The transcript levels in irradiated wild-type embryos are lower and similar to nonirradiated levels at stage 12 (E,F,Q,R) and are comparable to nonirradiated levels at stage 14 (I,J,U,V). Transcript levels are significantly higher in irradiated dmt embryos (D,H,L,P,T,X) than nonirradiated embryos (C,G,K,O,S,W) at all stages. All panels are lateral views. Due to differences in expression patterns between hid and rpr, different focal planes of similarly staged embryos are shown.