TABLE 1.
Phenotypic Analysis of dmt Alleles, Allelic Combinations, and Various Recombinant Lines Used in the Study
| Salivary Duct1 |
Trachea2 |
|||||||
|---|---|---|---|---|---|---|---|---|
| Gap(s) in ID |
No gaps | n | Gap(s) in DT |
No Gaps | n | |||
| Severe | Moderate | Severe | Moderate | |||||
| wt | 0% | 0% | 100% | 102 | 0.9% | 14.2% | 84.9% | 106 |
| dmt589 | 0% | 6.6% | 93.4% | 106 | 37.9% | 3.9% | 58.3% | 103 |
| dmt589/dmt3999 | 11.9% | 21.1% | 67.0% | 109 | 77.4% | 1.9% | 20.8% | 106 |
| dmt589/dmtI184 | 10.5% | 17.1% | 72.4% | 105 | 27.0% | 9.0% | 64.0% | 111 |
| dmt589/DfGB104 | 0.9% | 6.6% | 92.5% | 106 | 32.5% | 8.8% | 58.8% | 114 |
| dmt3999 | 25.2% | 18.7% | 56.1% | 123 | 96.3% | 1.9% | 1.9% | 107 |
| dmtI184 | 31.9% | 26.9% | 41.2% | 119 | 35.8% | 14.2% | 50% | 120 |
| dmt3999/dmtI184 | 31.4% | 18.6% | 50.0% | 102 | 57.5% | 6.6% | 35.8% | 106 |
| dmt3999/DfGB104 | 16.8% | 25.2% | 58.0% | 143 | 68.9% | 5.8% | 25.2% | 103 |
| dmtI184/DfGB104 | 19.6% | 19.6% | 60.8% | 102 | 36.7% | 8.3% | 55.0% | 109 |
| DfGB104 | 32.7% | 20.9% | 46.4% | 110 | 97.1% | 1.0% | 1.9% | 103 |
| DfH99, dmtI184 | 5.3% | 15.9% | 78.8% | 113 | 15.6% | 10.8% | 73.5% | 102 |
| dmt3999, btl-GAL43/dmt3999 | 27.1% | 22.7% | 50.2% | 207 | 62.1% | 8.0% | 29.9% | 264 |
| dmt3999, UAS-dmt4/dmt3999 | 21.3% | 25.0% | 53.7% | 108 | 96.6% | 1.4% | 1.9% | 208 |
| UAS-P357; dmt3999/dmt3999, btl-GAL43 | 1.9% | 11.1% | 87.0% | 108 | 29.3% | 13.8% | 56.9% | 123 |
| dmt3999, UAS-dmt4/dmt3999, btl-GAL43 | 2.8% | 5.6% | 91.7% | 108 | 9.9% | 7.2% | 82.9% | 111 |
| dmt3999, tub-GAL45/dmt3999 | 6.9% | 19.6% | 73.5% | 102 | 94.2% | 3.9% | 1.9% | 103 |
| dmt3999, UAS-dmt4/dmt3999, tub-GAL45 | 0.0% | 0.8% | 99.2% | 126 | 64.7% | 5.2% | 30.2% | 116 |
| sens-GAL47; dmt3999/dmt3999, UAS-dmt4 | 6.9% | 8.8% | 84.3% | 102 | 95.1% | 2.0% | 2.9% | 102 |
| elav-GAL48;; dmt3999/dmt3999, UAS-dmt4 | 8.4% | 0.9% | 90.6% | 107 | 97.1% | 1.0% | 1.9% | 103 |
| btl-GAL4 > UAS-hid6 | 11.4% | 26.7% | 61.9% | 105 | 100% | 0% | 0% | 122 |
| btl-GAL4 > UAS-grim7 | 4.0% | 36.6% | 59.4% | 101 | 100% | 0% | 0% | 104 |
| btl-GAL4 > UAS-rpr7 | 0% | 0% | 100% | 100 | 94.2% | 3.8% | 1.9% | 104 |
| btl-GAL4 | 1.0% | 1.0% | 98% | 104 | 10.9% | 8.2% | 80.9% | 110 |
| UAS-grim7 | 0% | 0% | 100% | 104 | 5.7% | 1.9% | 92.5% | 106 |
| UAS-rpr7 | 0% | 0% | 100% | 104 | 5.0% | 5.0% | 90% | 100 |
| UAS-hid6 | 1% | 0% | 100% | 106 | 17.9% | 5.4% | 76.8% | 112 |
The phenotypes were quantified either as severe (missing whole ID and/or gaps in both IDs), moderate (a single gap in one ID), or normal (no gaps).
The phenotypes were quantified either as severe (multiple and/or large gaps), moderate (single small gaps), or normal (no gaps).
dmt3999, btl-GAL4 is one recombinant line of btl-GAL4 on third chromosome with dmt3999.
dmt3999, UAS-dmt collectively refers to two different recombinant lines of UAS-dmt on third chromosome with dmt3999 and to one recombinant line of UAS-GFP-dmt on second chromosome with dmt3999. We saw no differences in the percent rescue with the tagged versus untagged versions of UAS-dmt.
dmt3999, tub-GAL4 collectively refers to two different recombinant lines of tub-GAL4 on third chromosome with dmt3999.
UAS-hid refers to two different insertion lines of UAS-hid one on X chromosome and one on the second chromosome.
All four insertions are on the second chromosome.
elav-GAL4 insertion is on the X chromosome.