Figure 5. Repeated exposure to LPS increases densities of CD150 and numbers of CD150^Hi HSC in bone marrow.

Marrow was harvested from mice given daily injections of saline or LPS for four-six weeks. Subsets of HSC in the LSK gate illustrated in Supplemental Fig. 1B were resolved on the basis of CD48 and/or Flt3 expression. A, Densities of CD150 are reflected in mean fluorescence intensities (MFI). B, HSC identified as CD150⁺ CD48⁻ LSK were subdivided into CD150^Lo and CD150^Hi fractions (See Supplemental Fig. 1B) that were enumerated in control and LPS treated mice.