. 2010 Sep 30;1:37–42.

Table 1.

Pharmacological properties of NK-PLA₂-A, NK-PLA₂-B, KTX-A, KTX-B and PLA₂-KTX complexes. Values are mean ± SD of four experiments. Values in the same row within each experiment followed by different superscripts are significantly different (P<0.05).

Properties	Control	PLA₂-A	KTX-A	PLA₂-A: KTX-A	PLA₂-B	KTX-B	PLA₂-B: KTX-B
Anticoagulant activity¹	87 ± 3.0^a	99 ± 4.0^b	89 ± 3.0^a	99 ± 3.0^b	100 ± 5.0^b	90 ± 3.0^a	99 ± 4.0^b
Antibacterial activity²	0	0	0	0	0	0	0
Cytotoxicity³
(a) VERO	0.3 ± 0.08^a	1.3 ± 0.2^b	0.8 ± 0.1^c	8.2 ± 0.3^d	1.0 ± 0.1^b	0.6 ± 0.1^c	6.8 ± 0.6^d
(b) Sf9 (n)	0.8 ± 0.1^a	4.9 ± 0.5^b	1.1 ± 0.1^c	12.2 ± 1.3^d	3.8 ± 0.4^b	0.9 ± 0.1^c	10.2 ± 1.1^d
(c) SF9 (in)	2.5 ± 0.1^a	8.1 ± 0.6^b	3.3 ± 0.4^c	20.1 ± 0.8^d	6.9 ± 0.4^b	3.9 ± 0.4^c	18.5 ± 1.3^d
(d) Tn (n)	1.8 ± 0.2^a	7.9 ± 0.5^b	4.8 ± 0.3^c	21.0 ± 1.1^d	6.6 ± 0.5^b	4.1 ± 0.3^c	20.2 ± 1.2^d
Direct hemolysis⁴ (time in min)
30	0^a	0^a	0^a	1.5 ± 0.5^b	0^a	O^a	1.0 ± 0.04^b
60	0^a	0.21 ± 0.02^b	0.11 ± 0.01^c	6.3 ± 1.1^d	0^a	0.1 ± 0.01^c	4.1 ± 0.1
90	0.1^a	0.73 ± 0.01^b	0.45 ± 0.02^c	14.3 ± 2.1^d	0^a	0.41 ± 0.02^c	13.2 ± 1.3^d
120	0.2^a	3.3 ± 0.2^b	1.5 ± 0.1^c	32.0 ± 3.4^d	0.4 ± 0.03^e	1.4 ± 0.1^c	29.1 ± 2.1^d

^aPlasma Ca-clotting time in seconds caused by 4.0μM PLA₂/KTX/PLA₂-KTX complex.

^bPost 12hr incubation with NK-PLA₂ / KTX / PLA₂-KTX at a concentration of 500μM

^cPercent of cell death post 3hr treatment with NK-PLA₂/KTX/PLA₂-KTX at a concentration of 250nM.

^dPercent of hemoglobin released from 5% (v/v) RBC suspension post incubation for indicated time period by 250nM PLA₂/KTX/PLA₂-KTX complex post 60min incubation at 37°C. Cent-percent hemolysis was achieved by adding 1% (v/v) Triton X-100 to RBC suspension.