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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Cell Signal. 2011 Mar 6;23(7):1144–1152. doi: 10.1016/j.cellsig.2011.02.009

Fig. 6. Inhibition of JNK prevents hexadecenal-induced cytoskeletal reorganization and apoptosis.

Fig. 6

A, NIH3T3 cells were cultured in 8-chamber slides for 24 h. Cells were serum deprived for 3 h in the absence or presence of a JNK inhibitor V (JNK Inhib, 20 μM), then cells were treated with vehicle (a-c) or hexadecenal (25 μM) (d-i) for 30 min. F-actin was labeled with Phalloidin-Rhodamine and cells were counter stained with DAPI. B, Cells were cultured in 10-cm culture dishes for 24 h. Cells were serum deprived for 3 h in the presence or absence of a JNK inhibitor V (JNK inhib, 20 μM), then cells were treated with vehicle or hexadecenal (25 μM) for 3 h. Cells were harvested and whole cell extracts were immunoblotted with PARP, phospho-C-Jun or actin antibodies. Immunoblots are representative of at least three independent experiments.