Table. Partial gene locations of virus isolated from dogs with respiratory disease and relatedness to other pneumoviruses*.
| Region sequenced† | PCR primers (5′ → 3′) and aa target sequences in pneumoviruses | % Identity to MPV, nt, aa† | % Identity to HRSV, nt, aa‡ | % Identity to HMPV, nt, aa§ |
|---|---|---|---|---|
| N gene,¶ nt 852–1182 (331 bp) |
P1R: ggaactcgggggcgaayttytccat
target: MEKFAPEFH
N276F: tccgtgcaggccgaratggarcarg
target: SVQAEMEQV |
96.4, 96.3 |
68.5, 70.1 |
56.5, 54.1 |
| L gene, no. 1,¶ nt 1143–1452 (310 bp) |
L428F: ccggatcttcggccayccnatggt
target: RIFGHPMV
L538R: ttcttaggaggggagatggcyttrtcrtt
target: NDKAISPPKN |
95.8, 97.1 |
62.1, 55.3 |
58.9, 47.6 |
| L gene, no. 2,¶ nt 1962–2511 (550 bp) | L698F: catcaccgacctgtccaagttyaaycargc target: ITDLSKFNQA L894R: ttgaagtcgtccaggatggtrttdatcca target: WINTILDDFK | 96.7, 99.5 | 68.4, 71.6 | 66.4, 68.3 |
*MPV, murine pneumovirus; nt, nucleotide; aa, amino acid; HRSV, human respiratory syncytial virus; HMPV, human metapneumovirus; N, nucleocapsid; R, reverse primer; F, forward primer L, polymerase. †Based on comparison with MPV strain J3666, GenBank accession no. NC_006579. ‡Based on comparison with HRSV, GenBank accession no. NC_001803. §Based on comparison with HMPV, GenBank accession no. NC_004148. ¶The N gene fragment is entered under GenBank accession no. GU247050; L gene fragments nos. 1 and 2 are entered under GenBank accession no. GU247051.