Table 1. Primers used in this study of invasive Streptococcus pneumoniae isolates, Atlanta, Georgia, USA, 1994–2006*†.
| Region | Primer | Sequence (5′ → 3′)‡ |
|---|---|---|
| Erm cassette | erm_F | gctctagaCGTTAGATTAATTCCTACCAGTGAC |
|
|
erm_R |
gctctagaCTCCATTCCCTTTAGTAACGTGTAAC |
| PI-2 | pepT_F | TAAGAAGCGGTCCAAGAGATTTGG |
| hemH_R | AATAATGGGGCTCCAAAATCAAGC | |
| sipA_up_F | CTCTAGGAGGGATCTTCTTTATCATC | |
| sipA_do_R | CTACAGCCGTTGTTCGATTGTCC | |
| PilA_del_3 | gcaattgcccgggcctagCCTGTATAGGGATGGTTCCAAAAG | |
| PilA_del_2 | ctaggcccgggcaattgcGCTGGGGGCAGATGATG | |
|
|
Rlr_up_F |
CTTCCACGAAGTTCTTTCAATGG |
| PI-1 | Rlr_do_R | GTCTTAGAATATCATGGTTTACGTGC |
| Rlr_srtC_F | GGGGAAGATTATGCGACCTT | |
| Rlr_srtD_R | GCTTGGCTCTGCACGGTGCC |
*PI, pilus islet. †Not included are primers used for sequencing and multilocus sequence typing. ‡Lowercase letters represent bases added for mutagenesis; uppercase letters represent bases complementary to S. pneumoniae.