Figure 1. Validation for SREBP-2 antibody and chromatin supplemented by chow and chow with LE.

Immunofluorescence of SREBP-2 in mouse liver from normal chow (A) or chow supplemented with lovastatin and ezetimibe (LE) for one week (B). Livers pieces were embedded in paraformaldehyde, sectioned, and stained with a rabbit anti-SREBP-2 polyclonal antibody (affinity purified), followed by fluorescein-labeled goat anti-rabbit IgG. Magnification, 400× bar shows scale for 10 μm. (C) Hepatic nuclear extracts were prepared from mice fed chow and LE diets. (D) Gene-specific ChIP analysis using chromatin from the LE group that was immuno-enriched with an antibody to SREBP-2 (Filled bars) or a control IgG (open bars). Gene specific primers for the indicated promoter regions were used in the qPCR. Data are mean ± SEM; n = 3 for 3 separate experiments.