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. Author manuscript; available in PMC: 2012 Apr 6.
Published in final edited form as: Cell Metab. 2011 Apr 6;13(4):367–375. doi: 10.1016/j.cmet.2011.03.005

Figure 4. SREBP-2 regulates autophagy-related gene expression.

Figure 4

(A) 293 cells were transfected with an siRNA (10 nM) for a negative control siRNA (lanes 1 and 2) or siRNA targeting hSREBP-2 (lanes 3 and 4). Twenty-four hours after transfection, cells were cultured in 5% lipoprotein deficient media (LPDS) lanes 2 and 4 or LPDS plus sterols (12 μg/ml cholesterol and 1 μg/ml 25-hydroxycholesterol) for 24 h. and harvested for RNA or total protein (Sanchez et al., 1995). (A) mRNA levels for LC3B, ATG4B, ATG4D, HMGCR and GAPDH were analyzed by RT-qPCR and relative expression is plotted. (B) Whole cell lysates were analyzed for SREBP-2 or LC3 protein levels by immunoblotting. Data are representative of 3 separate experiments. Data are mean ± SEM; n = 3 for 3 separate experiments. HMGCR, HMG Co-A reductase; BP2i, SREBP-2 siRNA; NCi, negative control siRNA.