Table I.
Primer pairs were designed to amplify the predicted SREBP-2 binding region from 12 genes predicted to harbor the SRE element and two separate sets of chromatin prepared from mice fed the LE diet and enriched with the SREBP-2 antibody versus control IgG were analyzed by qPCR. Hmgcr and L32 served as positive and negative controls respectively.
Gene | Fold Change (SREBP-2/IgG) | |
---|---|---|
Exp1 | Exp 2 | |
Atg4b | 3.35* | 6.28* |
Atg4d | 1.7* | 7.28* |
Dhps | 8.11* | 3.91* |
Mtf2 | 2.1# | 3.29* |
Mthfr | 9.51* | 8.69* |
Mtmr2 | 2.46# | 2.91* |
Ncoa3 | 5.86# | 3.8* |
Pds5b | 3.09# | 2.25 |
Sirt1 | 2.93# | 1.48 |
Snx13 | 9.1* | 1.58* |
Crk | 1.67* | 2.21# |
Hiat1 | 9.23* | 4.86* |
Hmgcr | 7.16* | 20.16* |
L32 | 0.89 | 1.38 |
p< 0.005,
p<0.05