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. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: J Bioanal Biomed. 2010 Oct 6;2:113–120. doi: 10.4172/1948-593x.1000033

Figure 1.

Figure 1

Transfection of macrophages with the eIF2α-S51A mutant attenuated E−/B48 lipoprotein-induced activation of the eIF2α–ATF4 signaling pathway. Raw 264.7 cells were stably transfected with a plasmid vector expressing eIF2α-S51A (S51A) or an empty pEGFP vector. The cells were incubated at 37°C for 24 hrs with 20 μg/ml of E+/B48 or E−/B48 lipoproteins or culture medium alone (control). The levels of total eIF-2α and ATF4 proteins and the levels of phosphorylated eIF-2α (eIF2α-p) were determined by western blot analysis and expressed relative to β-tubulin levels. Values represent the mean ± SEM of five separate experiments. *P<0.05 as compared with the control, and †P<0.05 as compared with cells transfected with empty pEGFP vector and treated with E−/B48 lipoproteins.