Skip to main content
. Author manuscript; available in PMC: 2011 Oct 6.
Published in final edited form as: J Bioanal Biomed. 2010 Oct 6;2:113–120. doi: 10.4172/1948-593x.1000033

Figure 4.

Figure 4

Transfection of macrophages with the eIF2α-S51A mutant attenuated E−/B48 lipoprotein-induced changes in the expression of lysosomal hydrolases. (A and B) Raw 264.7 cells stably transfected with eIF2α-S51A (S51A) or empty pEGFP vector were treated at 37°C for 12 hrs with 20 μg/ml of E+/B48 or E−/B48 lipoproteins or culture medium alone. The protein levels of LAL, Cath B, and β-tubulin were measured by immunoblot analysis. LAL and Cath B levels are expressed as the percentage of their immunoblot intensities relative to β-tubulin. (C and D) Raw 264.7 cells were transfected with a plasmid vector expressing LAL (LALTg) or an empty pEGFP vector. The cells were incubated at 37°C for 12 hrs with 20 μg/ml of E−/B48 lipoproteins or culture medium alone. Cellular cholesterol contents were determined. Values represent the mean ± SEM of five experiments. *P<0.05 as compared with the control, and †P<0.05 as compared with cells transfected with empty pEGFP vector and treated with E−/B48 lipoproteins.