Figure 1.

Bright field microscopy demonstrating morphology of RLT-PSCs in (A) the quiescent state, and activated by (B) exposure to PCC conditioned media, or (C) growth on a tissue-culture treated plate. Fluorescent microscopy demonstrating (D) quiescent PSC lipid droplets (red), GFAP Expression (green), and nucleus (blue); (E) quiescent PSC lipid droplets (red), α-SMA (green), and nucleus (blue); and (F) activated PSC loss of lipid droplets (red), α-SMA Expression (green), and nucleus (blue). Cells in images A, B, D, E, and F were plated on collagen I coated coverslips, while those in image C were plated on tissue-culture treated plates. All cells were plated under serum-free conditions.