Table 2.

Serial section staining scheme. IHC analysis conducted on human tissue samples from patients with pancreatitis, PDAC, or disease-free subjects was used to confirm and localize expression of selected proteins in relation to PSCs. PSCs were visualized by staining serial sections of tissue using PSC markers α-SMA and vimentin. To differentiate PSCs from endothelial cells, which also express α-SMA and vimentin, the endothelial cell marker, CD-31, was used. Therefore PSCs could be readily identified by the presence of α-SMA and vimentin and the absence of CD-31. Staining of serial sections was organized such that IHC staining of proteins of interest, listed in black, were performed in a tissue section immediately adjacent to a set of PSC markers, italicized.

Slide #	Protein
1	SERPINI2
2	α-SMA
3	CD-31
4	vimentin
5	PKM2
6	TGFBI
7	α-SMA
8	CD-31
9	vimentin
10	EZR
11	LGALS3BP
12	α-SMA
13	CD-31
14	Vimentin
15	UCHL1
16	NME1
17	α-SMA
18	CD-31
19	vimentin
20	ANXA2