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. Author manuscript; available in PMC: 2012 Apr 26.
Published in final edited form as: Biochemistry. 2011 Mar 17;50(16):3282–3287. doi: 10.1021/bi2001664

FIGURE 2.

FIGURE 2

CD spectra of apo and ligand-bound wild-type caspase-6 reflect the change in structure of the 60’s and 130’s helix upon ligand binding. In contrast the CD spectra of caspase-7 and -3 do not reflect a change in helical content. The network-disrupting variant (E53K/E63V) shows a similar change in helical content upon ligand binding to wild-type caspase-6. The helix breaking variants C7HB and C3HB show a much decreased difference in CD spectra between apo and ligand bound, suggesting that the apo state is less helical in solution than wild-type caspase-6.