. Author manuscript; available in PMC: 2012 Apr 26.

Published in final edited form as: Biochemistry. 2011 Mar 17;50(16):3282–3287. doi: 10.1021/bi2001664

Table 1.

Kinetic parameters and melting temperatures for wild-type (WT) caspases, the network-disrupters and helix-breakers.

	K_M (μM)	k_cat (s⁻¹)	Apo T_m (°C)	Active site bound T_m (°C)	ΔT_m (°)

WT casp-6^a	66 ±7	0.81 ±0.01	77 ± 0.5	80.3 ± 0.4	3.3
WT casp-3	5^a	9.1^b	83	>90	>7
WT casp-7^c	23 ±2	0.35 ±3 ×l0⁻³	59.8 ±0	77.7 ± 3.8	17.9
E53K	100 ±28	0.7 ±0.07	77 ±0.1	77.2 ±0.4	0.2
E63V	121 ±24	0.78 ±0.08	74.6 ±1.5	77.5 ±0.1	2.9
E53K+E63V	68 ±16	0.74 ±0.06	75.5 ±0.2	78.3 ± 0.8	2.8
C3HB (A132G, K133P)	46 ± 3.5	0.84 ± 0.06	70.7 ±1.0	76.5 ±0.7	5.8
C7HB (A132G, E135P)	79 ±13	1.2 ±0.03	73.1 ±0.5	77.8 ±0.3	4.7

K_m, k_cat, and T_m were measured in triplicate from samples independently prepared on three separate days.

Reported in (9).

Reported in (17).

Reported in(18).