Cytokine mRNA expression of IEL, MLN lymphocytes, and splenocytes from mice infected orally with E. cuniculi. IEL, MLN lymphocytes, and splenocytes from C57BL/6 mice (four mice per group) were isolated at different time points p.i. (days 0, 3, 7, 14, 21, 28, 50). mRNA expression of IFN-γ and IL-10 was assayed by RT-PCR. The differences in the transcriptional levels for the genes are expressed relative to day 0 (assigned as 1). The cDNA concentration examined at each time point was standardized to the hypoxanthine phosphoribosyltransferase mRNA level (data not shown). The experiment was performed twice with similar results, and the data is representative of one experiment.