Figure 3.

Generation of OL progenitors in cultures initiated from E12.5 Fgfr1^−/− or Fgfr2^−/− mutant forebrains is reduced in a manner similar to that in vivo. A, Dissociated cell cultures from E12.5 forebrains of Fgfr1^−/− or Fgfr2^−/− mutants and littermate controls, were immunolabeled as a function of days in vitro (DIV) for specific markers of pre/early progenitors (Olig2 at 3 DIV and PDGFRa at 6 DIV), late progenitors (O4 at 9 DIV), and OLs (HPC7 at 12 DIV). B, The numbers of Olig2+, PDGFRa+, and O4+ cells were counted from each culture and expressed as a percentage of control showing significantly reduced numbers of these cells in both mutants compared with littermate controls. Scale bar, 50 μm. Error bars represent SEM (*p < 0.01, **p < 0.02). Cultures from three to six pups were analyzed for each group.