Indirect immunofluorescence labeling of different cell types was carried out using Alexa Fluor 594 or Alexa Fluor 488 conjugated secondary antibodies. DAPI (blue) was used to stain nuclei. Panels A to H show proteins found to be expressed at higher levels in ESCs. Panel A to G includes immunocytochemical staining for proteins encoded by DNMT3B (blue-green nuclei), DNMT3A (blue-green nuclei), GSN (red cytoplasm), UTF1 (blue-green nuclei), BGN (red secretory), LGALS1 (green cell surface), CTNNB1 (green cell surface) and HELLS (blue-green nuclei), respectively.