Table 2.
A partial list of proteins expressed at higher levels in ECCs as compared to ESCs
| Gene Symbol | RefSeq Accession | Protein Name | Peptides ¶ | ESC/ ECC iTRAQ ratio | Sample |
|---|---|---|---|---|---|
| PARP1 | 4501955 | poly (ADP-ribose) polymerase family, member 1 | 25 | 0.3* | Non-cytosolic |
| PHGDH | 23308577 | phosphoglycerate dehydrogenase | 32 | 0.1* | Whole cell |
| TPI1 | 4507645 | triosephosphate isomerase 1 | 19 | 0.2* | Cytosolic |
| FKBP4 | 4503729 | FK506-binding protein 4 | 17 | 0.3* | Cytosolic |
| FLNC | 116805322 | gamma filamin | 15 | 0.3* | Cytosolic |
| GANAB | 38202257 | alpha glucosidase II alpha subunit isoform 2 | 17 | 0.5* | Non-cytosolic |
| LOC654188 | 113429184 | PREDICTED: similar to peptidylprolyl isomerase A isoform 1 | 5 | 0.2* | Non-cytosolic |
| AHCY | 9951915 | S-adenosylhomocysteine hydrolase | 10 | 0.1* | Whole cell |
| STRAP | 20149592 | serine/threonine kinase receptor associated protein | 9 | 0.3* | Cytosolic |
| DHX9 | 100913206 | DEAH (Asp-Glu-Ala-His) box polypeptide 9 | 12 | 0.4* | Cytosolic |
| PRKDC | 126032350 | protein kinase, DNA-activated, catalytic polypeptide 2 | 17 | 0.2* | Whole cell |
| RAN | 5453555 | ras-related nuclear protein | 13 | 0.1* | Whole cell |
| SNRNP200 | 40217847 | activating signal cointegrator 1 complex subunit 3-like 1 | 9 | 0.4* | Whole cell |
| LRPPRC | 31621305 | leucine-rich PPR motif-containing protein | 10 | 0.3* | Whole cell |
| KHSRP | 4504865 | KH-type splicing regulatory protein (FUSE binding protein 2) | 12 | 0.2* | Whole cell |
| RPL7A | 4506661 | ribosomal protein L7a | 6 | 0.3* | Whole cell |
| KRT18 | 4557888 | keratin 18 | 22 | 0.2** | Whole cell |
| HSPB1 | 4504517 | heat shock 27kDa protein 1 | 10 | 0.1** | Whole cell |
| TCP1 | 57863257 | T-complex protein 1 isoform a | 7 | 0.2** | Whole cell |
| PCNA | 4505641 | proliferating cell nuclear antigen | 3 | 0.2** | Whole cell |
| DEK | 4503249 | DEK oncogene | 3 | 0.1** | Whole cell |
| PCMT1 | 4885539 | protein-L-isoaspartate (D-aspartate) O-methyltransferase | 4 | 0.1** | Whole cell |
| FSCN1 | 4507115 | fascin 1 | 4 | 0.4** | Non-cytosolic |
| PRDX2 | 32189392 | peroxiredoxin 2 isoform a | 4 | 0.4** | Cytosolic |
| NHP2L1 | 51317376 | NHP2 non-histone chromosome protein 2-like 1 | 3 | 0.2** | Whole cell |
| PSMB2 | 4506195 | proteasome beta 2 subunit | 3 | 0.1** | Whole cell |
| MAGOHB | 8922331 | mago-nashi homolog 2 | 5 | 0.3** | Cytosolic |
| PRDX4 | 5453549 | thioredoxin peroxidase | 7 | 0.2** | Whole cell |
| PA2G4 | 124494254 | ErbB3-binding protein 1 | 2 | 0.1** | Whole cell |
| SFRS4 | 21361282 | splicing factor, arginine/serine-rich 4 | 1 | 0.1** | Whole cell |
| HNRNPH1 | 5031753 | heterogeneous nuclear ribonucleoprotein H1 | 13 | 0.2** | Whole cell |
| CSTB | 4503117 | cystatin B | 3 | 0.1** | Whole cell |
| SLC25A5 | 4502099 | solute carrier family 25, member 5 | 11 | 0.1*** | Whole cell |
| DDOST | 20070197 | dolichyl-diphosphooligosaccharide-protein glycosyltransferase | 2 | 0.3*** | Whole cell |
| AHNAK | 61743954 | AHNAK nucleoprotein isoform 1 | 3 | 0.3*** | Non-cytosolic |
| CLTC | 4758012 | clathrin heavy chain 1 | 23 | 0.4*** | Whole cell |
| CALU | 4502551 | calumenin precursor | 3 | 0.2*** | Non-cytosolic |
| PSMC1 | 24430151 | proteasome 26S ATPase subunit 1 | 6 | 0.3*** | Whole cell |
| MLLT4 | 90819237 | Myeloid/lymphoid or mixed lineage leukemia, translocated to 4 | 1 | 0.4 | Non-cytosolic |
| PEBP1 | 4505621 | prostatic binding protein | 7 | 0.3 | Whole cell |
| MFGE8 | 5174557 | milk fat globule-EGF factor 8 protein | 2 | 0.4 | Whole cell |
| MIF | 4505185 | macrophage migration inhibitory factor | 4 | 0.1 | Whole cell |
| CD99 | 4505183 | CD99 antigen | 1 | 0.3 | Cytosolic |
| CTNNBL1 | 18644734 | beta catenin-like 1 | 1 | 0.3 | Whole cell |
| PODXL | 66277202 | podocalyxin-like precursor isoform 1 | 3 | 0.3 | Non-cytosolic |
| GDF3 | 10190670 | growth differentiation factor 3 precursor | 1 | 0.3 | Non-cytosolic |
| THY1 | 19923362 | Thy-1 cell surface antigen | 1 | 0.4 | Whole cell |
| PTPN9 | 4506301 | protein tyrosine phosphatase, non-receptor type 9 | 1 | 0.5 | Non-cytosolic |
| SPARC | 4507171 | secreted protein, acidic, cysteine-rich (osteonectin) | 1 | 0.5 | Cytosolic |
| MAPK1 | 66932916 | mitogen-activated protein kinase 1 | 1 | 0.5 | Whole cell |
| SART1 | 10863889 | squamous cell carcinoma antigen recognized by T cells 1 | 1 | 0.5 | Cytosolic |
| TEX264 | 7706708 | testis expressed sequence 264 | 1 | 0.5 | Whole cell |
| CSNK2A1 | 4503095 | casein kinase II alpha 1 subunit isoform a | 4 | 0.4 | Cytosolic |
| DDB1 | 13435359 | damage-specific DNA binding protein 1 | 8 | 0.3 | Whole cell |
| MGB2 | 11321591 | high-mobility group box 2 | 1 | 0.3 | Non-cytosolic |
(p value
*
<0.001,
**
<0.01 and
***
<0.05, unused score >1.3,
¶
Number of peptides with >95% confidence score used for quantitation)