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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Gene Ther. 2010 Nov 11;18(4):344–353. doi: 10.1038/gt.2010.139

Figure 1.

Figure 1

MKP-1 gene transfer specifically dephosphorylated MAPKs in rat macrophages after LPS stimulation. Rat macrophages (NR8383 cells) were transduced with Ad.MKP-1 (moi 300), or Ad.LacZ (moi 300), or treated with equal volume of HEPES buffered saline for 48 hours. Cells were either unstimulated or stimulated with 1μg/ml of LPS (from A. actinomycetemcomitans) for various time periods (10, 30, 60, 120, 180, and 300 minutes). The MKP-1, p-JNK (p54, p46), p-p38 MAPK, p-ERK (p44, p42), p-38 MAPK, and p-NF-κB (p65, p105) protein expression were examined by western blot assays. The p-38 MAPK served as a loading control. (a) MKP-1 protein expression in rat macrophages. (b) Phosphorylated MAPKs protein expression in rat macrophages. (c) Phosphorylated NF-κB p65 and p105 protein expression in rat macrophages. The data is a representative from three separate experiments.

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