Figure 3.
MKP-1 gene transfer inhibited osteoclasts formation in bone marrow (BM) cells from MKP-1 KO mice after LPS stimulation. BM cells were extracted from either eight-week-old MKP-1 KO mice or WT mice. Cells were transduced with Ad.MKP-1 or control Ad.LacZ (moi 25) for 48 hours and then incubated with MEM-alpha medium containing 10% FBS, 2 mM glutamine, 50ng/ml recombinant mouse M-CSF (R&D systems) with or without LPS (1μg/ml, from A. actinomycetemcomitans) for 8 days. Osteoclasts were stained by tartrate resistant acid phosphatase (TRAP) staining. (a) Multinucleated osteoclasts (arrows shown) in bone marrow cells from MKP-1 KO mice and WT mice. (b) Number of osteoclasts (more than three nuclei) per well (96-well plate) in bone marrow cells from MKP-1 KO mice and WT mice (n=4, **p<0.01, ***p<0.001).