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Flow cytometric quantitation of H2O2 production and accumulation of resting DCs as compared with PM-exposed DCs. Data are geometric MFI ± SD as a function of DCF fluorescence (oxidized DCFHDA). The respiratory burst of DCs derived from Nrf2+/+ and Nrf2−/− mice is shown following stimulation with PM (A) and as compared with the negative control carbon black (CB) particulates (B), bacterial LPS/endotoxin (C), and trimeric CD40L (D). ko, Knockout.
