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. Author manuscript; available in PMC: 2011 May 3.
Published in final edited form as: Mol Cancer Res. 2008 May;6(5):743–750. doi: 10.1158/1541-7786.MCR-07-0095

FIGURE 1.

FIGURE 1

HMGA2 expression is increased in lung cancer cell lines from metastatic disease.

A. Western blot analysis shows increased HMGA2 in the human lung cancer cell lines H1299 (metastatic non-small cell carcinoma), SK-MES-1 (metastatic squamous cell carcinoma), H82 (metastatic small cell carcinoma) compared with the normal human bronchial epithelium cell line BEAS-2B. In H358 cells, derived from non-metastatic, non-small cell bronchioloalveolar carcinoma, HMGA2 protein levels are similar to that of the BEAS-2B immortalized control cells line.

B. Quantitative RT-PCR analysis shows increased HMGA2 mRNA in H1299, SK-MES-1, and H82 metastatic lung cancer cells compared to BEAS-2B cells, consistent with the Western blot analysis (A). Values shown are fold-differences of RNA expression compared to BEAS-2B cells (arbitrarily assigned a value of 1); results represent the mean +/− the standard deviation from two independent experiments performed in triplicate.