Figure 5.

Apoptosis machinery is required for dendrite pruning but not dendrite degeneration. Animals of the same genotypes were assayed for both dendrite degeneration after severing and dendrite pruning during metamorphosis. Control genotypes are as in Figure 2. In addition to mCD8–GFP, experimental animals expressed Gal4-controlled p35, DIAP1, or a hairpin RNA to target the dronc transcript. For severing experiments, larvae were mounted, and a dendrite from ddaC was severed with a pulsed UV laser. After 18 h recovery on food, the presence of dendrite remnants was scored. For pruning experiments, animals were mounted for imaging 18 h APF, and presence of ddaC dendrite remnants was scored. WT, Wild type.