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Specific players required for dendrite pruning are not required for dendrite degeneration. The same strategy shown in Figure 5 was used to test the role of IK2, Kat–60L1, and Mical in dendrite degeneration, again compared with pruning in cells of the same genotype. RNAi to reduce each of these proteins was performed by expressing UAS-controlled hairpin RNAs. The presence of dendrite remnants in ddaC was scored 18 h after severing or 18 h APF.
